Lecture 19 Carrier Protein Structure and Recognition in Peptide Biosynthesis

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چکیده

Carrier proteins in polyketide synthase (PKS) and nonribosomal peptide synthase (NRPS) systems play an important role in syntheses of polyketides and nonribosomal peptides respectively (Figure 1). Carrier proteins are relatively small in size (80−100 amino acids; 8−10kDa) compared to other catalytic domains in a synthase system. The key role of carrier proteins is to serve as an attachment site for monomers (acyl, aminoacyl, or aryl compounds), and intermediates during the sequential synthetic reactions, shuttling intermediates between catalytic modules during the synthesis. Both monomers and intermediates are attached to a carrier protein at a thiol group of phosphopantetheine cofactor that is covalently attached to a carrier protein. Since there are three kinds of possible building blocks for these two enzyme systems, carrier proteins are categorized into groups accordingly: Acyl Carrier Proteins (ACPs), Peptidyl Carrier Proteins (PCPs), and Aryl Carrier Proteins (ArCPs). Basic reaction steps are shared among different systems. These sequential reaction steps include activation of a monomer, and condensation between an activated monomer and a growing chain.

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تاریخ انتشار 2007